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Mary S. Maish, M.D., M.P.H.

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Tricuspid Valve Biopsy: A Potential Source of Cardiac Myofibroblast Cells
for Tissue-Engineered Cardiac Valves which includes:

Tricuspid valve leaflet biopsies are a potential source for harvesting cells to be used in the development of a tissue-engineered cardiac valve. Exact link.

In English this is:

Dr. Maish has started some exciting medical research in the area of "harvesting" living cells from your body that can be used to "grow" actual tissue to use instead of the artificial material used in "valve replacement!"

Dr. Mary Maish is the surgeon who turned around Jean Ross' life!  Click here to read the story about the miracle recovery from esophagus cancer for Jean.

Medical School Education
Institution: Rush Medical College
Year: 1993
Board Certified

Postdoctoral Education:
 

Institution Level (Intern,
Resident, Fellow)		 Department Year
Harvard University Masters Public Health 2001
Rush Medical College Internship General Surgery 1993-1994
Michigan State University Residency General Surgery 1994-1995
University of Pennsylvania affiliated St. Luke's Hospital Residency General Surgery 1995-1999
Baylor College of Medicine/MD Anderson Cancer Center Residency Cardiothoracic Surgery 2001-2003
Brown University, Roddy Scholar in Cardiothoracic Research Fellowship Cardiothoracic Surgery 1999-2001
University of Southern California Fellowship Thoracic and Foregut Diseases 2003-2004

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Research Interests

  • Esophageal Cancer and Barrett's esophagitis

  • Population based studies on esophageal cancer and Barrett's esophagitis

  • Domestic and International health policy

Clinical Interests

  • Esophageal Cancer

  • Esophageal Disorders

  • GERD

  • Lung Cancer

  • Minimally invasive and robotic surgery

Hospital Staff Appointments

  • Surgical Director of the UCLA Center for Esophageal Disorders

Academic/Teaching Appointments

  • Assistant Professor of Surgery, Division of Thoracic Surgery

Memberships in Scholarly or Professional Societies

  • American Medical Association

  • American College of Surgeons

Source

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Tricuspid Valve Biopsy: A Potential Source of Cardiac Myofibroblast Cells
for Tissue-Engineered Cardiac Valves

Mary S. Maish(1), Diane Hoffman-Kim, Paula M. Krueger, Jason M. Souza, James J. Harper, III, Richard A. Hopkins

Department of Surgery, Brown University School of Medicine and The Collis Cardiac Surgery Research Laboratory of the Rhode Island Hospital, Providence, Rhode Island, USA
(1)Present address: Department of Cardiothoracic Surgery, Baylor College of Medicine,
Houston, Texas, USA
 
Background and aims of the study: As progress is made in the development of a tissue-engineered cardiac valve, the need for a reliable cell source is particularly important. A technique has been developed for the reliable biopsy of tricuspid valve leaflets. Expanding the harvested cells in culture is feasible and provides a source of leaflet cells that are structurally and functionally similar to the pulmonary and aortic valve leaflet cells that they may replace.
Methods: Thirteen sheep underwent tricuspid valve biopsy. Transthoracic echocardiography (TTE) was performed to evaluate function and guide the subsequent biopsy. Myofibroblasts were isolated from the biopsy samples, expanded in culture through 10 passages, and evaluated with immunocytochemistry for valve cell markers. Two animals were sacrificed acutely, two animals died during the immediate postoperative period, and nine animals survived for four weeks or more.
Results: All preoperative and pre-explantation echocardiograms were normal. Both animals sacrificed acutely showed that the tricuspid valve leaflet was indeed biopsied with this technique.

 		 Two perioperative deaths occurred; one animal died secondary to injury of the chorda tendinea with subsequent destruction of the posterior leaflet; another died from disruption of the superior vena cava that led to irreversible cardiac tamponade. At sacrifice (2 to 17 weeks), all other animals showed intact tricuspid valves with normal leaflet anatomy. All cultured biopsies generated myofibroblasts that were immunocytochemically positive for alpha smooth muscle actin, chondroitin sulfate, vimentin and fibronectin.
Conclusion: Biopsy of the tricuspid valve to obtain recipient cardiac valve leaflet cells is possible, and the technique is simple and reliable. Biopsy of the leaflet does not compromise function. Interstitial cells can be harvested and expanded in culture. Cellular structure and function is preserved and is similar to that of other cardiac leaflet cells. Tricuspid valve leaflet biopsies are a potential source for harvesting cells to be used in the development of a tissue-engineered cardiac valve.

[In English, this means:  Dr. Maish has started some exciting medical research in the area of "harvesting" living cells from your body that can be used to "grow" actual tissue to use instead of the artificial material used in "valve replacement!"]
 

The Journal of Heart Valve Disease 2003;12:264-269
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